AME prepress abstract  -  DOI: https://doi.org/10.3354/ame01912

Genome analysis of Marinobacter phage AS1 suggests its close interactions with host Marinobacter sp

S. Aparna, A. Parvathi*, R. a. m. A. S. Pradeep, T. Sime-Ngando

*Email: parvathi@nio.org

ABSTRACT: Marinobacter comprises a widespread and ecologically significant genus of marine bacteria with vast metabolic capabilities. However, very few reports on phages infecting this genus exist. In this study, we isolated and characterized a linear dsDNA genome of Marinobacter phage AS1, infecting Marinobacter sp. strain, D1S9. The phage is a member of Podoviridae family having a genome size of ~37 kb and a 57% GC content with no significant overall similarity to any of the available viral whole genome sequences in the database. The genome which encodes for 52 protein coding genes with no tRNA genes, contains 21 protein coding genes with assigned putative functions. High degree of recombination events with other members of the family Caudovirales is evident from the mosaic pattern of inheritance of genes such as major capsid protein, terminase large sub unit, portal protein and integrase. Presence of integrase gene, DNA methyl transferases and the occurrence of AS1 sequences within the whole genome sequences of some members of the host taxon were suggestive of its intense association with the host.