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AME prepress abstract   -  DOI:

Assessment of viral assemblages in different types of wetlands water in northeast China using RAPD-PCR

Yan Sun, Junjie Liu, Qin Yao, Jian Jin, Xiaobing Liu, Guanghua Wang*

*Corresponding author:

ABSTRACT: Although viruses are the most abundant and ubiquitous biological entities in various ecosystems, few investigations of viral communities in wetlands have been performed. To address this, water samples from 6 wetlands were randomly collected across northeast China; viruses in the water were concentrated by sequential tangential flow filtration, and the viral communities were assessed through randomly amplified polymorphic DNA-PCR (RAPD-PCR) with 4 decamer oligonucleotide primers. Principal coordinate analysis and hierarchical clustering analysis of the DNA fingerprints showed that viral community compositions distinctly differed among the different water samples: communities in the 2 coastal wetlands were more similar to each other than to those in the 4 freshwater wetlands. The Shannon-Weaver index (H) and evenness index (E) of the RAPD-PCR fingerprint banding patterns generated with the different primers also differed among the 6 wetlands. A Mantel test revealed that the changes in viral communities in wetland water were most closely related to the water NH4+-N and inorganic C content, followed by the total K, total P, total C and NO3-N contents. DNA sequence analysis of the excised bands revealed that virus sequences account for ~40% of all sequences. Among the hit viral homologs, the majority of sequences belonged to the Microviridae. Moreover, variance partitioning analysis showed that viral community contributed 24.58% of the bacterial community variation, and water environmental factors explained 30.56% of the variation, indicating that the profile of bacterial community composition was strongly affected by both viral community and water variables. This work provided an initial outline of the viral communities from different types of wetland in northeast China and improves our understanding of the viral diversity in these ecosystems.