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AB prepress abstract   -  DOI: https://doi.org/10.3354/ab00726

Soya isoflavones, genistein and daidzein, inducing differential transcriptional modulation in the ovary and testis of the zebrafish, Danio rerio

Carmen Sarasquete*, María Úbeda-Manzanaro, Juan B. Ortiz-Delgado

*Corresponding author:

ABSTRACT: Most of the effects of phytochemical isoflavones have focused on endocrine disruptions, and especially on oestrogenic imbalances, but little is known about their mechanisms of action directed towards other molecular signals, such as transcriptional coregulators and choriolytic enzymatic pathways, which also participate in important reproduction purposes. In males and females of zebrafish, soya isoflavones (genistein and daidzein, at 10 mg / L, for 15 days) modulated in a variable and differential way the basal expression levels of oestrogen receptor transcripts (ERβ), which decreased in the ovary due to exposure to genistein. Conversely, this isoflavone increased the basal expression levels of the hatching enzyme (HE1), in both gonads. On the other hand, isoflavone daidzein increased the basal expression levels of the bromodomain testis-specific gene (BRDT) in the male gonad, but not in the ovary. Both isoflavones have also differentially modulated (up-down regulations) the basal expression patterns of the three molecular signals studied, in the other regions of the body (e.g., head, digestive system, skeletal musculature). Despite all these transcriptional imbalances, both phytoestrogens did not modify gonadal histomorphology, nor the baseline histochemical pattern of proteins, carbohydrates and glycoconjugates distributed in vitelline structures, and in developing and maturing germ cells of Danio rerio.