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AB prepress abstract   -  DOI:

A simple method for long-term recording of the electrocardiogram in prawns

Han-Tso Lin*, Tsen-Chien Chen, Jun-Ping Chang, Zin-Yan Chou, Shian-Yu Chiou, Meng-Li Tsai

*Corresponding author:

ABSTRACT: This paper presents a novel approach to the long-term recording of electrocardiogram (ECG) signals in prawns, including a 3-lead electrode, a lightweight external wires assembly, a waterproof electrode-wire junction and a standardized implanting procedure. The proposed low-cost device is easily fabricated, even by untrained undergraduate students using common laboratory materials. Consistent ECG recordings were obtained over a period of 72 h in experiments on 6 freely moving prawns (Macrobrachium rosenbergii). A subsequent experiment conducted continuously for 38 d revealed that, despite gradual attenuation, the ECG signals could still be identified (signal-to-noise ratio ≥ 3). The prawns survived implantation and later underwent natural molting, whereupon the electrode was successfully re-implanted. The proposed electrode is a valuable tool by which to gain insight into the long-term physiological state of crustaceans.