DAO prepress abstract  -  DOI: https://doi.org/10.3354/dao03390

Investigating the natural resistance of blackfoot pāua Haliotis iris to abalone viral ganglioneuritis using whole transcriptome analysis

Matthew J. Neave, Serge Corbeil*, Kenneth A. McColl, Mark St. J. Crane

*Email: s.corbeil@csiro.au

ABSTRACT: The natural resistance of New Zealand blackfoot pāua Haliotis iris to infection by haliotid herpesvirus-1 (HaHV-1) and to the disease abalone viral ganglioneuritis was investigated in experimentally challenged pāua using high throughput RNA-Seq. HaHV-1-challenged pāua up-regulated broad classes of genes that contained chitin-binding peritrophin-A domains, which seem to play diverse roles in the pāua immune response. The pāua also up-regulated vascular adhesion protein-1 (VAP-1), an important adhesion molecule for lymphocytes, and chitotriosidase-1 (CHIT-1), an immunologically important gene in mammalian immune systems. Moreover, several blood coagulation pathways were dysregulated in the pāua, possibly indicating viral modulation. We also saw several indications that neurological tissues were specifically affected by HaHV-1, including the dysregulation of beta-1,4-N-acetylgalactosaminyltransferase (B4GALNT), GM2 ganglioside, neuroligin-4 and the Notch signalling pathway. This research may support the development of molecular therapeutics useful to control and/or manage viral outbreaks in abalone culture.