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Diseases of Aquatic Organisms

    DAO prepress abstract   -  DOI: https://doi.org/10.3354/dao03407

    First identification of fish pathogens Tenacibaculum dicentrarchi and T. soleae in Tasmania and multiplex PCR for these organisms and T. maritimum

    Teresa K. Wilson*, Marianne Douglas, Vivianne Dunn

    *Corresponding author:

    ABSTRACT: This work describes a new polymerase chain reaction (PCR) assay for the rapid identification of the fish pathogens Tenacibaculum dicentrarchi, T. maritimum and T. soleae, three organisms which can cause tenacibaculosis in farmed salmonids. The selected primers amplified a 688 bp fragment for T. dicentrarchi, a 288 bp fragment of the T. maritimum, and an 183 bp fragment of the T. soleae 16S rRNA genes. The PCR assay was shown to be both specific and sensitive with a detection limit of approximately 50 fg DNA for each species in the presence of competing DNA. The multiplex PCR allowed for the detection of each pathogen from pure or mixed cultures where the different Tenacibaculum species can be difficult to distinguish phenotypically. Our results indicated that the specific primers and PCR method developed here provide sensitive and fast detection of T. dicentrarchi, T. maritimum or T. soleae alone or in combination.