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DAO
Diseases of Aquatic Organisms

    DAO prepress abstract   -  DOI: https://doi.org/10.3354/dao03413

    Toxicological, hematological and immunological effects of levamisole and ivermectin diet supplementation in Colossoma macropomum (Serrasalmidae)

    Lígia Carvalho Dias Oliveira, Alexandre Renato Pinto Brasiliense, Márcia Kelly Reis Dias, Eliane Tie Oba Yoshioka, Marcos Tavares-Dias*

    *Corresponding author:

    ABSTRACT: This study investigated the toxicological, hematological and immunological effects of diets supplemented with levamisole and ivermectin on Colossoma macropomum. Fish were fed for 24, 96 and 240 h with diets containing 300, 600, 900 and 1200 mg kg-1 diet of levamisole. None of the levamisole concentrations caused either mortality or behavioral alterations among the fish over 10 days of feeding. In comparison, a single feeding of ivermectin at 4500, 9000, 13500 or 18000 mg kg-1 diet caused 100% mortality among the fish within 10 h. There were no alterations in erythrocyte parameters or albumin in any treatments with levamisole, after 24, 96 and 240 h of feeding. At concentrations of 900 and 1200 mg kg-1 diet levamisole caused decreases in the albumin-to-globulin ratio, when compared to the control group and the 300 and 600 mg kg-1 diet treatment groups. Levels of glucose and total plasma protein were higher in the fish fed with 600, 900 and 1200 mg kg-1 diet than in the controls and the 300 mg kg-1 diet treatment group. Ten days after receiving feed with levamisole, globulin levels were higher in the fish that received 600 mg kg-1 diet when compared to the controls. Respiratory burst activity of leukocytes also increased in the fish supplemented with 900 and 1200 mg kg-1 of levamisole in diet, in comparison with the controls and other treatments. This is first study to investigate how diets supplemented with these drugs effect on Colossoma macropomum. Our research indicates that all the levamisole concentrations tested can be used in the diet of C. macropomum for antiparasitic treatments against helminth species and that dietary treatments with levamisole can stimulate components of the innate immune system.