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Diseases of Aquatic Organisms

    DAO prepress abstract   -  DOI:

    Efficacy of silver nanoparticles to control flavobacteriosis caused by Flavobacterium johnsoniae in common carp Cyprinus carpio

    Mohamed Shaalan*, Boglárka Sellyei, Mansour El-Matbouli, Csaba Székely

    *Corresponding author:

    ABSTRACT: Flavobacterial infections are among the causes of fish losses in farms with the emergence of antibiotic-resistant isolates. Silver nanoparticles (AgNPs) are known for their potent antimicrobial activity against differnet types of bacteria. In this study, we evaluated the antibacterial properties of AgNPs (diameter = 23 nm) against Flavobacterium johnsoniae infection in common carp Cyprinus carpio. The assays included both in vitro and in vivo antibacterial tests besides the evaluation of their cell toxicity effect to the cell fish lines. The in vitro results revealed potent inhibitory effect of AgNPs on the bacterial growth of F. johnsoniae with minimum inhibitory concentration of 34 µg ml–1. Fish cell (epithelioma papulosum cyprini and koi carp fin) viability was 95-100% after exposure to 500 ng ml–1 and lower concentrations of AgNPs. In exposure experiment, mortality rates reduced from 45% in infected non-treated group to 30% and 15% in intraperitoneal injection and immersion-treated groups, respectively. Both treated groups did not show any clinical signs or histopathological lesions. The single-dose treatment with AgNPs during early infection with F. johnsoniae aided in minimizing fish losses.