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Diseases of Aquatic Organisms

    DAO prepress abstract   -  DOI:

    Metabolic responses of shrimp Palaemonetes sinensis to isopod Tachaea chinensis parasitization

    Yingdong Li, Weibin Xu, Xin Li, Zhibin Han, Ruiyang Zhang, Xiaodong Li, Qijun Chen*

    *Corresponding author:

    ABSTRACT: Tachaea chinensis, a parasitic isopod, negatively affects the production of several commercially important shrimp species in China. The mechanism of parasite–host interaction cannot be accurately described by transcriptomic and proteomic approaches individually. Here, to achieve a broad coverage of primary metabolite changes in Chinese grass shrimp Palaemonetes sinensis following T. chinensis parasitization, comparative metabolite profiling was performed. Sixty-six metabolites significantly differentially accumulated between the control and infected groups were annotated; of these, 19 were upregulated and 47 were downregulated after T. chinensis infection. Moreover, the Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis revealed that 10 pathways were significantly enriched. The protein digestion and absorption pathways were highly enriched, followed by the mineral absorption, aminoacyl-tRNA biosynthesis, biosynthesis of amino acids, and metabolic metabolism pathways. Parasitization by T. chinensis enhanced the glycolytic pathway and tricarboxylic acid (TCA) cycle in P. sinensis, thereby releasing more energy for swimming, foraging, and evading predation. Glucogenic amino acids such as alanine, histidine, glutamine, and proline were consumed to generate glutamate and enhance the TCA cycle. Nucleotide-related metabolic pathways were downregulated, possibly because T. chinensis can secrete molecules to degrade nucleotides and inhibit hemostasis and inflammatory responses. These results suggest that the isopod parasite can increase the host’s metabolic burden by enhancing the host’s TCA cycle and secreting molecules to degrade host proteins, thereby enabling it to feed on the host and inhibit an inflammatory response. The results will be a valuable contribution to understanding the metabolic responses of crustaceans to isopod parasitism.