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Diseases of Aquatic Organisms

    DAO prepress abstract   -  DOI: https://doi.org/10.3354/dao03463

    Truncated infectious hematopoietic necrosis virus G protein effect on survival, immune response, and disease resistance in rainbow trout

    Xiaojing Hua, Ying Feng, Xin Guan, Yanxue Wang, Ying Zhou, Xuanyu Ren, Dechuan Li, Shuai Gao, Jinshan Huang, Xueting Guan, Wen Shi, Min Liu*

    *Corresponding author:

    ABSTRACT: The major antigenic protein of infectious hematopoietic necrosis virus (IHNV) is the surface glycoprotein G, which contains neutralizing epitopes that induce the production of immune neutralizing antibodies. In this study, the IHNV G gene sequence was truncated according to bioinformatics principles and then recombinantly expressed via an E. coli expression system. We then assessed the specific antibody IgM levels of rainbow trout immunized with recombinant truncated G protein (emulsified with Freund's incomplete adjuvant), and showed that antibody IgM levels of immunized fish were significantly higher than in the control group. The mRNA expression levels of IFN1 and IL-8 were also up-regulated significantly (p < 0.01) in head kidneys and spleens of rainbow trout immunized by recombinant truncated G protein. Also, after challenge with wild-type IHNV HLJ-09 virus on day 28, rainbow trout immunized by recombinant truncated G protein showed cumulative survival rates of 60%. These results indicate that the truncated G protein of IHNV expressed by E. coli prokaryotic expression system can be used as a candidate immunogen for an IHNV subunit vaccine, which lays a theoretical foundation for study of further potential IHNV subunit vaccines.