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Diseases of Aquatic Organisms

    DAO prepress abstract   -  DOI: https://doi.org/10.3354/dao03488

    Isolation and maintenance of Batrachochytrium salamandrivorans cultures

    Kristyn A. Robinson, Kenzie E. Pereira, Molly C. Bletz, Edward Davis Carter, Matthew J. Gray, Jonah Piovia-Scott, John M. Romansic, Douglas C. Woodhams, Lillian Fritz-Laylin*

    *Corresponding author:

    ABSTRACT: Discovered in 2013, Batrachochytrium salamandrivorans (Bsal) is an emerging amphibian pathogen that causes ulcerative skin lesions and multifocal erosion. A closely related pathogen, Batrachochytrium dendrobatidis (Bd), has devastated amphibian populations worldwide, suggesting that Bsal poses a significant threat to global salamander biodiversity. To expedite research into this emerging threat, we seek to standardize protocols across the field so that results of laboratory studies are reproducible and comparable. We have collated data and experience from multiple labs to standardize culturing practices of Bsal. Here we outline common culture practices including a medium for standardized Bsal growth, standard culturing protocols, and a method for isolating Bsal from infected tissue.