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Diseases of Aquatic Organisms

    DAO prepress abstract   -  DOI: https://doi.org/10.3354/dao03491

    Enhancement of shrimp health and immunity by oral administration of diets supplemented with combined probiotics: application to Vibrio parahaemolyticus infections

    Chettupon Pooljun, Sunisa Daorueang, Wattana Weerachatyanukul, Sataporn Direkbusarakom, Pitchanee Jariyapong*

    *Corresponding author:

    ABSTRACT: The application of probiotics for disease control in aquaculture is now a convincing approach to replace antibiotics, which can cause adverse effects in aquatic animals and humans. In this study we combined 2 probiotics, Lactobacillus acidophilus and Saccharomyces cerevisiae, into 2 formulas (WU8 and WU9) and mixed these with a daily diet to feed juvenile shrimp which were then given a challenge infection of Vibrio parahaemolyticus, the causative agent of acute hepatopancreas necrosis disease (AHPND). The protective effects of probiotics against bacterial infection were investigated through histopathology of the hepatopancrease and immunological evaluation of shrimp. Both WU8 and WU9 probiotic mixtures (at 108 and 109 CFU kg diet–1) increased blasenzellen (B) hepatopancreatic epithelial cells and reduced pathology caused by AHPND. After 10 d of feeding, hemocyte parameters, including the total hemocyte count, % of granular hemocytes, and phenoloxidase activity, increased significantly and extended to 24 h post infection. Crustin and penaeidin 3 genes were also highly upregulated in hemocytes before and after 24 h of bacterial challenge and significantly upregulated in the hepatopancreas in all study periods. A significantly higher survival rate was observed in shrimp fed with the probiotic supplemented diet (>90%) in comparison to the control group (60%). In conclusion, probiotic mixtures of L. acidophilus and S. cerevisiae reduced hepatopancreas pathology and protected shrimp from a challenge with AHPND.