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Diseases of Aquatic Organisms

    DAO prepress abstract   -  DOI:

    First detection of infectious haematopoietic necrosis virus in farmed rainbow trout in North Macedonia

    Aleksandar Cvetkovikj, Vladimir Radosavljevic*, Argelia Cuenca, Biljana Strojmanovska, Jelena Maksimovic-Zoric, Iskra Cvetkovikj, Niels Jørgen Olesen

    *Corresponding author:

    ABSTRACT: Infectious haematopoietic necrosis virus (IHNV) is the causative agent of infectious haematopoietic necrosis, a disease of salmonids responsible for great economic losses. The disease occurs in most parts of the world where rainbow trout is reared but has not been previously reported in North Macedonia. In this study, 150 pooled samples in total, each consisting of organ mix of 10 freshly killed rainbow trout Oncorhynchus mykiss, were collected from 50 trout farms by the Food and Veterinary Agency (FVA) of North Macedonia, as part of the Annual surveillance plan for IHN and VHS control. Screening of samples was done by cell culture and qRT-PCR. All 150 tested samples were VHSV qRT-PCR negative. Two samples from different trout farms were IHNV qRT-PCR positive. On cell culture, one IHNV qRT-PCR positive sample caused cytopathic effect (CPE) after 2 passages on the EPC cell line. The virus, isolated from an asymptomatic rainbow trout fry, was identified by qRT-PCR and designated as MAKIHNV1. The phylogenetic reconstruction indicates that the isolated virus belongs to the European E genogroup, more specifically within the E-1 clade, and is similar to the German, Italian and Iranian isolates. This study has revealed for the first time the presence of IHNV in rainbow trout in North Macedonia. However, it is not possible to make interpretations about the source of infection from the phylogenetic analysis and the origin of MAKIHNV1 remains unclear.