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Diseases of Aquatic Organisms

    DAO prepress abstract   -  DOI: https://doi.org/10.3354/dao03538

    Association between ALFPm3 single nucleotide polymorphism and white spot syndrome virus resistance in black tiger shrimp Penaeus monodon

    Chettupon Pooljun, Pitchanee Jariyapong, Sataporn Direkbusarakom, Sudarat Saeng-ngern, Takashi Sakamoto, Suwit Wuthisuthimethavee*

    *Corresponding author:

    ABSTRACT: Here single nucleotide polymorphisms (SNPs) were associated with white spot syndrome virus (WSSV) resistance in black tiger shrimp (Penaeus monodon). SNPs were identified by single-strand conformation polymorphism (SSCP) screening and DNA sequencing of shrimp sampled from 3 families (100 shrimp/family) challenged with WSSV. Shrimp that died over the 14-day challenge trial were assigned as susceptible, with those remaining alive on Day 14 assigned as resistant. To compare SNPs, 10 samples from the susceptible and resistant groups each comprising DNA pooled from 3 shrimp were amplified by PCR using primers to 12 selected genes and screened by SSCP. SNP polymorphisms were only identified in the anti-lipopolysaccharide factor 3 (ALFPm3) gene product. Analysis of complete ALFPm3 gene sequences confirmed the existence of 3 SNPs (g.934-C>G, g.1186-A>G, and g.1898-C>G) that were polymorphic between the susceptible and resistant groups. Further analyses of these 3 SNPS using specific tetra-primer ARMS-PCR primer sets associated them, and particularly the g.1186-A>G SNP, with WSSV resistance. This SNP thus has potential for use as a DNA marker for selecting for WSSV resistance in P. monodon breeding programs.