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Diseases of Aquatic Organisms

    DAO prepress abstract   -  DOI:

    Molecular and histopathological identification of Infectious Myonecrosis virus (IMNV) and Infectious Hypodermal and Hematopoietic necrosis virus (IHHNV) in cultured penaeid shrimp in Egypt

    Salah M. Aly*, Shimaa M. Mansour, Randa Y. Thabet, Mahmoud Mabrok*

    *Corresponding author:

    ABSTRACT: The present study aimed to diagnose IMNV and IHHNV among cultured penaeid shrimp Penaeus semisulcatus (n = 120) collected from private farms, located in 2 different Provinces (Damietta and North Sinai Province) along the Mediterranean coast, Egypt. The collected shrimp were subjected to clinical examination, histopathology, molecular characterization, and phylogenetic analysis. Most of the infected shrimp with IMNV showed a distinctive appearance of cooked shrimp and white necrosis on distal abdominal segments and tail fans. Simultaneously, IHHNV infected cases displayed opaque abdominal muscles, white milky to buff mottling spots on the shell, and a pathognomonic runt-deformity syndrome. The histopathological examination of infected specimens showed muscular edema, hemocyte infiltration, deformities, Zenker's necrosis, and eosinophilic intra-nuclear inclusion bodies (Cowdry type A). PCR results gave predictable amplicons size of 139 and 81 bp and confirmed the presence of IMNV and IHHNV with a total prevalence of 37.5 and 25%, respectively. Homology search by BLAST analysis proved that the retrieved isolates putatively belonged to IMNV and IHHNV based on 96.3–>97% nucleotide identity to the corresponding ORF gene of each virus. The phylogenetic analysis clearly showed genetic identity and cross-lineage between our isolates and other isolates of Egypt, the USA, Brazil, Indonesia, China, Korea, Taiwan, and Ecuador. In conclusion, gross inspection and histopathology may aid in the diagnosis of viral diseases; however, molecular tools are indispensable for confirming a possible infection. The current study recommends strict regulations during live shrimp transportation and implementing health control certificates overall imports and exports, especially in developing countries, including Egypt.