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Diseases of Aquatic Organisms

    DAO prepress abstract   -  DOI: https://doi.org/10.3354/dao03570

    Molecular identification of burn spot disease syndrome agent Fusarium avenaceum in populations of Turkish crayfish Pontastacus leptodactylus

    Büşra Taştan, Süleyman Akhan*

    *Corresponding author:

    ABSTRACT: In this study, we aimed to isolate and identify the causative agent of burn spot disease syndrome from crayfish populations within Turkish water bodies. Crayfish (Pontastacus leptodactylus) samples were captured from Bayramşah Reservoir, Karaidemir Reservoir and Keban Reservoir (Turkey). Fungi samples were taken from symptomatic crayfish and cultured in potato dextrose agar and DNAs were extracted. Species-specific PCR primers (FAF1 and FAR) were used in order to amplify ITS region of fungal DNA. Sequences obtained from the amplified regions were analyzed by using GenBank and identification was performed accordingly. As a result, the molecular diagnosis of the fungi samples was achieved and the pathogen was identified as Fusarium avenaceum. In addition, a challenge test with healthy crayfish was conducted to evaluate the pathogenicity of fungi. F. avenaceum was reisolated from experimentally infested individuals and thusly we confirmed the disease. It was concluded that F. avenaceum causes burn spot disease syndrome in P. leptodactylus