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Diseases of Aquatic Organisms

    DAO prepress abstract   -  DOI:

    Outbreak of vibriosis associated with Vibrio parahaemolyticus in the mud crab Scylla paramamosain cultured in China

    Jiasong Xie, Han Mei, Shan Jin*, Lingfei Bu, Xinyi Wang, Chunlin Wang, Qingsong Zhao, Rongrong Ma, Suming Zhou

    *Corresponding author:

    ABSTRACT: In this study, a Gram-negative bacterium was isolated from diseased Scylla paramamosain and tentatively named strain QX17. The bacterial isolate was identified as Vibrio parahaemolyticus based on morphological and biochemical characteristics, and molecular identification with the 16S rRNA and HSP60 genes. In the challenge experiment, Scylla paramamosain injected intramuscularly with the V. parahaemolyticus isolate developed similar pathological signs to the naturally diseased mud crab. The infection experiment also showed that the median lethal dosage (LD50) for QX17 was 4.79 × 102 CFU/g (crab weight). Histopathological analysis of the diseased mud crab infected with V. parahaemolyticus showed deformation and basement membrane rupture of hepatopancreatic tubules in the hepatopancreas, and disordered and broken muscle fiber in the muscle. Antimicrobial susceptibility tests revealed that QX17 was highly sensitive to most of the tested aminoglycosides, tetracyclines, and quinolones. To the best of our knowledge, this is the first study reporting isolation and antibiotic sensitivities of V. parahaemolyticus from cultured mud crab. The discovery of V. parahaemolyticus in cultured mud crab not only adds to the growing list of emerging pathogens in crab aquaculture in China, but also highlights the necessity of developing early detection strategies and appropriate interventions to reduce the damage caused by V. parahaemolyticus in mud crab aquaculture.