DAO 145:51-61 (2021)  -  DOI: https://doi.org/10.3354/dao03594

ABSTRACT: White spot syndrome virus (WSSV) is the most destructive virus among invertebrates. In this study, we analyzed the immune response after WSSV infection in Pacific white shrimp Litopenaeus vannamei using isobaric tags for relative and absolute quantitation (iTRAQ). We identified 325 differentially expressed proteins (DEPs) in the hepatopancreas of L. vannamei. Among them, 212 were up-regulated proteins, and several of them might be related to immunity (e.g. arginine kinase and peroxiredoxin). Of the 113 down-regulated proteins, some were related to immunity (e.g. cathepsin C and cathepsin L) and others to the antioxidant defense process (e.g. glutathione peroxidase and catalase). One down-regulated DEP (C7M84_014268) and 3 up-regulated DEPs (C7M84_003456, C7M84_020702, and C7M84_007135) were randomly selected and analyzed using parallel reaction monitoring. This study is an important step for a comprehensive understanding of the immune relationship between L. vannamei and WSSV and provides valuable information for the prevention of viral diseases in the crustacean aquaculture industry.

KEY WORDS: Isobaric tags for relative and absolute quantitation · iTRAQ · Litopenaeus vannamei · Proteomics · Parallel reaction monitoring · PRM · WSSV