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Diseases of Aquatic Organisms

    DAO prepress abstract   -  DOI: https://doi.org/10.3354/dao03604

    Visceral mycobacteriosis in amphibians from Brazilian Caatinga Region

    Drausio Honorio Morais*, Marianna Vaz Rodrigues, Robson Waldemar Ávila, Reinaldo José da Silva

    *Corresponding author:

    ABSTRACT: Emerging infectious diseases in wild animals related to humans have received greater attention in recent years. Mycobacteriosis is a bacterial disease of animal and human importance. Mycobacterium gordonae infects the skin and internal organs of free ranging amphibians and is considered the least pathogenic member of Mycobacteriaceae to humans. However, there is still lack of information about its infection and pathogenesis in wild amphibians. A total of 1306 amphibian specimens belonging to six families, 12 genera, and 21 species were collected and dissected during a helminthological survey from seven municipalities in southern Ceará state, Caatinga region, Northeast Brazil. From these, 17 specimens (0.76%) distributed in two families and four species [Leptodactylus macrosternum (n = 2), L. vastus (n = 10), Pseudopaludicola pocoto (n = 2), and Rhinella jimi (n = 3)] presented infections that consisted in calcification nodules in the coelomic cavity, kidney, liver, lung, gut, and pancreas. The nodules were examined by histopathology and polymerase chain reaction (PCR). The bacteria were identified as M. gordonae by molecular analyses. Infected animals presented with hepatocellular vacuolar degeneration, karyolysis, and karyorrhexis, hepatic portal congestion, hemorrhage, mononuclear cellular infiltration, melanomacrophages centers hyperplasia and granulomas in varying stages of development with intralesional acid fast bacilli. This study is the first report of M. gordonae in these amphibian species, in which results of molecular analyses confirmed the presence of M. gordonae in natural environments and histopathology confirmed the typical lesion of mycobacteriosis in amphibians from Northeastern Brazil.