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Diseases of Aquatic Organisms

    DAO prepress abstract   -  DOI: https://doi.org/10.3354/dao03622

    Isolation, characterization and biocontrol efficacy of a T4-like phage virulent to multidrug-resistant Enterobacter hormaechei

    Wei Lin, Dengfeng Li*, Mingming Gao, Weinan Qin, Lihua Xu, Lingting Pan, Wencai Liu, Hang Fan, Zhiqiang Mi, Yigang Tong

    *Corresponding author:

    ABSTRACT: Enterobacter hormaechei is an important emerging pathogen, often exhibiting resistance to multiple clinically important antibiotics. In this study, E. hormaechei was found to be lethal to fish for the first time. Bacteriophages are considered potential treatments for bacterial infections. A lytic phage vB_EhoM-IME523 infecting multidrug-resistant E. hormaechei was isolated from hospital sewage. IME523 exhibits T4-like morphology containing a prolate icosahedron head with a length of 110 ± 1.89 nm, a width of about 82 ± 0.75 nm and a contractile tail of ca. 110 ± 0.91 nm in length. The complete genome length of phage IME523 is 172763 bp with a G + C content of 39.97%. The whole genome sequence of IME523 has a 93.10% average nucleotide identity (ANI) and a 53.3% in silico DNA-DNA hybridization (isDDH) value with the closest related Enterobacter phage vB_EclM_CIP9. ANI and isDDH values between IME523 and other phages were lower than 78 and 22%, respectively. IME523 and CIP9 formed a monophyletic branch in the phylogenetic tree based on terminase large subunit, DNA polymerase protein and whole genome phylogenetic analysis. Results suggest that IME523 is a novel species in the Tevenvirinae subfamily and it presents a novel genus together with CIP9. No IME523 ORF was found to be associated with virulence factors, antibiotic resistance genes. IME523 showed promising protection to zebrafish and brocade carp against E. hormaechei challenge.