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Diseases of Aquatic Organisms

    DAO prepress abstract   -  DOI: https://doi.org/10.3354/dao03665

    First record of isolation and characterization of Vibrio sinaloensis from diseased orange-spotted grouper, Epinephelus coioides

    Xue Zhang, Jingfeng Sun*, Yonglong Zhu, Zhuoran Han, Xiucai Hu, Aijun Lv, Yongjun Guo

    *Corresponding author:

    ABSTRACT: A dominant bacterium, ZYL-12, isolated from the liver of a diseased orange-spotted grouper Epinephelus coioides, was identified as Vibrio sinaloensis, based on phenotypic and molecular analysis. The median lethal dosage of ZYL-12 was calculated as 1.6 × 105 CFU g–1 fish weight. The infection experiment indicated that ZYL-12 caused noticeable histological lesions to the liver, kidney and spleen of the fish. Growth characteristics showed that ZYL-12 possessed strong environmental adaptability. This note is the first report about the pathogenicity of V. sinaloensis isolated from diseased fish.