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Diseases of Aquatic Organisms

    DAO prepress abstract   -  DOI:

    Metabolic changes in antennal glands of Caribbean spiny lobsters Panulirus argus infected by Panulirus argus virus 1 (PaV1)

    Ioreni Margarita Hernández-Velázquez, Jesús Alejandro Zamora-Briseño, Gloria Ivonne Hernández-Bolio, Emanuel Hernández-Nuñez, Enrique Lozano-Álvarez, Patricia Briones-Fourzán, Rossanna Rodríguez-Canul*

    *Corresponding author:

    ABSTRACT: Panulirus argus virus 1 (PaV1) (family Mininucleoviridae) causes a chronic and systemic infection to wild juvenile spiny lobsters Panulirus argus (Latreille, 1804), ending in death by starvation and metabolic wasting. In marine decapods, the antennal gland is involved in osmoregulation and excretion. In this compact organ, fluid is filtered from the hemolymph and ions are reabsorbed to produce a hypotonic urine. Although in infected individuals PaV1 is released with the urine, little is known regarding the metabolic effect of PaV1 in the antennal gland. The objective of this study was to perform a comparative evaluation of the metabolic profile of the antennal gland of clinically PaV1 infected, versus lobsters with no clinical signs of infection using proton nuclear magnetic resonance analysis. Overall, 48 compounds were identified, and the most represented metabolites were those involved in carbohydrate, amino acid, energy, and nucleotide metabolism. Most of the metabolites that were down-regulated in the infected group were essential and non-essential amino acids. Some metabolites involved in the urea cycle and carbohydrate metabolism were also altered. This study represents a first approach into the metabolic evaluation of the antennal gland. We broadly discuss the alterations in the content of several proteinogenic and non-proteinogenic amino acids and other key metabolites involved in the energetic and nucleotide metabolism.