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Diseases of Aquatic Organisms

    DAO prepress abstract   -  DOI:

    Epidemiological cut-off values for non-O1/non-O139 Vibrio cholerae disc diffusion data generated by standardised methods

    Peter Smith*, Laëtitia Le Devendec, Eric Jouy, Emeline Larvor, Jean Lesne, Alexander K. T. Kirschner, Carmen Rehm, Melanie Leopold, Sonja Pleininger, Florian Heger, Claudia Jäckel, Cornelia Göllner, Jonas Nekat, Jens Andre Hammerl, Sandrine Baron

    *Corresponding author:

    ABSTRACT: This work generates the data needed to set epidemiological cut-off values for disc-diffusion zone measurements of Vibrio cholerae. The susceptibility of 147 European isolates of non-O1/non-O139 Vibrio cholerae to nineteen antibiotics was established using a standardised disc diffusion method which specified incubation of Mueller Hinton agar plates at 35°C. Epidemiological cut-off values were calculated by analysis of the zone size data with the statistically based normalised resistance interpretation method. Cut-off values for seventeen agents were calculated by analysis of the aggregated data from all four laboratories participating in this study. The cut-off values calculated were ≥18 mm for amoxicillin-clavulanate, ≥18 mm for amikacin, ≥19 mm for ampicillin, ≥27 mm for cefepime, ≥31 mm for cefotaxime, ≥24 mm for ceftazidime, ≥24 mm for chloramphenicol, ≥31 mm for ciprofloxacin, ≥16 mm for erythromycin, ≥ 27 mm for florfenicol, ≥16 mm for gentamicin, ≥23 mm for imipenem, ≥25 mm for meropenem, ≥29 mm for nalidixic acid, ≥28 mm for norfloxacin, ≥13 mm for streptomycin and ≥23 mm for tetracycline. For the other two agents the data from one laboratory was excluded from the censored aggregation because the data from that laboratory was considered excessively imprecise. The cut-off values for these two agents calculated for the aggregation of the data from three laboratories were ≥23 mm for trimethoprim and ≥24 mm for trimethoprim-sulfamethoxazole. These zone size data will be submitted to CLSI and EUCAST for their consideration in setting international consensus epidemiological cut-off values for non O1/non-O139 Vibrio cholerae.