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Diseases of Aquatic Organisms

    DAO prepress abstract   -  DOI: https://doi.org/10.3354/dao03779

    Epidemiological cut-off values for Yersina ruckeri disc diffusion data generated by a standardised method

    Peter Smith*, Laƫtitia Le Devendec, Eric Jouy, Emeline Larvor, David Verner-Jeffreys, Andrew Wokorac Joseph, Elliot Stanton, Edel Light, Luana Cortinovis, Tobia Pretto, Amedeo Manfrin, Pierre-Marie Boitard, Matthieu Jamin, Nicolas Keck, Alain Le Breton, Benoit Thuillier, Christian Ravaille, Sandrine Baron

    *Corresponding author:

    ABSTRACT: In order to establish the meaning of data generated in antimicrobial agent susceptibility tests it is necessary to develop internationally harmonised interpretive criteria. Currently, such criteria have not been developed for data generated in studies of the susceptibility of the fish pathogen Yersinia ruckeri. This work generated the data that would be required to set epidemiological cut-off values for the susceptibility data of this species that had been generated using a standardised disc diffusion method that specified the use of Mueller Hinton agar and incubation at 22°C for 24-28h. Using this method sets of inhibition zones data for four antimicrobial agents were generated by three independent laboratories. The data from these laboratories were aggregated and analysed using the statistically-based normalised resistance interpretation. For ampicillin, florfenicol, oxytetracycline and trimethoprim-sulfamethoxazole the cut-off values calculated by this analysis were ≥16, mm, ≥23 mm, ≥24 mm and ≥30 mm respectively. Evidence is presented demonstrating that the data for these four agents was of sufficient quantity and quality that they could be used by the relevant authorities to set internationally harmonised, consensus epidemiological cut-off values for Y. ruckeri.