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Diseases of Aquatic Organisms

    DAO prepress abstract   -  DOI: https://doi.org/10.3354/dao03786

    Metabolomics captures the differential metabolites in the replication pathway of snakehead vesiculovirus regulated by glutamine

    Binbin Sun, Yulei Zhang, Keping Chen, Lindan Sun*

    *Corresponding author:

    ABSTRACT: Snakehead vesiculovirus (SHVV) is a negative-sense single-stranded RNA virus that infects snakehead fish. This virus leads to illness and mortality, which cause significant economic losses in the snakehead aquaculture industry. The replication and spread of SHVV in cells, which requires glutamine as a nitrogen source, is accompanied by alterations in intracellular metabolites. However, the metabolic mechanisms underlying the inhibition of viral replication by glutamine deficiency are poorly understood. This study utilized LC‒MS to measure the differential metabolites between Channel catfish (Parasilurus asotus) ovary cell line (CCO) infected with SHVV under glutamine-containing and glutamine-deprived conditions. Results showed that the absence of glutamine regulated four distinct metabolic pathways and influenced nine differential metabolites. Differential metabolites of PS(16:0/16:0), 5,10-methylene-THF, and PS(18:0/18:1(9Z)) were involved in amino acid metabolism. In the nuclear metabolism functional pathway, differential metabolites of guanosine were observed. In the carbohydrate metabolism pathway, differential metabolites of UDP-D-galacturonate were detected. In the signal transduction pathway, differential metabolites of SM(d18:1/20:0), SM(d18:1/22:1(13Z)), SM(d18:1/24:1(15 Z)), and sphinganine were found. Among them, PS(18:0/18:1(9Z)), PS(16:0/16:0), and UDP-D-galacturonate were involved in the synthesis of phosphatidylserine and glycoprotein. 5,10-methylene-THF provided raw materials for virus replication, and guanosine and sphingosine are related to virus virulence. The differential metabolites may collectively participate in the replication, packaging, and proliferation of the SHVV under glutamine deficiency. This study provides new insights and potential metabolic targets for combating SHVV infection in aquaculture through metabolomics approaches.