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Diseases of Aquatic Organisms

    DAO prepress abstract   -  DOI: https://doi.org/10.3354/dao03790

    Characterisation of common hypothetical surface peptides between protozoan parasites Perkinsus olseni originating from different geographical locations

    Eliot Hanrio*, Daniel Bogema, Kathleen Davern, Jacqueline Batley, Michael Clarke, Laila Abudulai, Anita Severn-Ellis, C├ęcile Dang

    *Corresponding author:

    ABSTRACT: Perkinsus olseni and P. marinus are classified as notifiable pathogens by the World Organisation for Animal Health and are known to cause perkinsosis in a variety of molluscs globally. Mass mortalities due to these parasites in farms and in the wild have been a recurrent issue. Diagnosis for these protozoans is currently done using Ray’s fluid thioglycollate medium method followed by optical microscopy or molecular assays. Both require a high level of skill and are time-consuming. An immunoassay method would make the diagnosis of perkinsosis quicker and cheaper. The present study used mass spectrometry-based proteomics to investigate common hypothetical surface peptides between different geographical isolates of P. olseni, which could be used to develop immunoassays in the future. Two peptides were identified: POLS_08089, which is a 42.7 KDa peptide corresponding to the 60S ribosomal subunit protein L4; and POLS_15916, which is a conserved hypothetical protein of 55.6 KDa. The identification of peptides may allow the development of immunoassays through a more targeted approach.