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Diseases of Aquatic Organisms

    DAO prepress abstract   -  DOI:

    Setting epidemiological cut-off values relevant to MIC and disc diffusion data for Aeromonas salmonicida generated by a standard method

    Peter Smith*, Elizabeth Buba, Andrew P. Desbois, Alexandra Adams, David Verner-Jeffreys, Andrew Joseph, Edel Light, Laƫtitia Le Devendec, Eric Jouy, Emeline Larvor, Pierre-Marie Boitard, Matthieu Jamin, Nicolas Keck, Alain Le Breton, Benoit Thuillier, Christian Ravaille, Thierry Morin, Sandrine Baron

    *Corresponding author:

    ABSTRACT: The Clinical and Laboratory Standards Institute has published epidemiological cut-off values for susceptibility data generated at 22°C and read after 44–48 h for florfenicol, oxolinic acid and oxytetracycline against Aeromonas salmonicida. The cut-off values for the minimum inhibitory concentration and disc diffusion were derived from data obtained by one laboratory and two laboratories respectively. This work reports the generation of susceptibility data from additional laboratories and the calculation of provisional cut-of values from aggregations of these data with previously published data. With respect to minimum inhibitory concentration data the provisional cut-off values, derived from aggregations of the data from four laboratories, were ≤4 µg/ml for florfenicol, ≤0.0625 µg/ml for oxolinic acid and ≤1 µg/ml for oxytetracycline. For disc diffusion data the provisional cut-off values derived from aggregations of the data from five laboratories were ≥30 mm for florfenicol, ≥32 mm for oxolinic acid and ≥25 mm for oxytetracycline. In addition, a cut-off value of ≥29 mm for ampicillin was derived from the aggregation of data from four laboratories.