ABSTRACT: Considerable attention has been directed to studying the infection dynamics of the fungal pathogen, Batrachochytrium dendrobatidis (Bd), affecting amphibians in the high elevations of the Neotropics. However, lowland forests of the same realm remain comparatively understudied in this context. Herein, we attempt to bridge this gap by measuring the prevalence of Bd via quantitative polymerase chain reaction (qPCR) in several anuran taxa inhabiting the Amazonian lowlands in the northeast of Ecuador. To this end, we sampled 207 anurans from 10 different families, 25 different genera, and 55 distinct host species originally collected in 2008. Taxonomy (at the family level), morphology (i.e., weight and snout-vent length), and life-long aquatic dependency of hosts (i.e., aquatic index) were also compiled to serve as potential predictors of Bd infection status. Our findings revealed a relatively high Bd prevalence of 58%, with 88% of sampled anuran families testing positive for the fungus at varying proportions. Model selection involving fitting and testing several different linear models, including mixed linear models, revealed a significant negative relationship between host weight and Bd infection status (p < 0.01). However, no significant associations were observed between taxonomy, aquatic dependency, snout-vent length, and Bd infections. In addition, we only detected the global panzootic lineage of Bd (Bd-GPL) and not the Bd-Asia-2/Bd-Brazil lineage via qPCR SNP genotyping. Our findings contribute to the understanding of Bd dynamics in the Neotropical lowlands and emphasize the need for future research on the ecological factors influencing Bd in the Amazon and their implications for amphibian conservation.