DAO prepress abstract   -  DOI: https://doi.org/10.3354/dao03833

ABSTRACT: Cyvirus cyprinidallo 2 (CyHV-2) is the pathogen of herpesviral hematopoietic necrosis (HVHN), that mainly infects goldfish (Carassius auratus) and crucian carp (Carassius carassius), and characterized by high infectivity and pathogenicity. The availability of rapid and convenient detection methods is essential for early detection of CyHV-2. A colloidal gold immunochromatographic strip using two specific anti-CyHV-2 mAbs has been developed and validated for rapid detection of CyHV-2. The test results can be viewed within 10 min. The detection limit of test strip was 2.08×10²TCID₅₀/mL, and it showed no cross-reactivity with other freshwater fish viral pathogen except KHV (Koi herpesvirus). The specificity of the strip was 100% when spleen and kidney tissues of CyHV-2 infected and healthy crucian carp were validated at the field level. The colloidal gold immunochromatographic strip will be an effective device for the rapid detection of CyHV-2 in the future.