AEI prepress abstract  -  DOI:

In vivo DNA damage in gill, haemolymph and muscle cells of whiteleg shrimp, Litopenaeus vannamei on exposure to organophosphorus pesticide

Ashwini P. Pawar, Sushant V. Sanaye, Soorambail Shyama, Rayadurga A. Sreepada*, Jacky Bhagat, Praveen Kumar, Rakhee D. Sinai Khandeparker


ABSTRACT: In response to growing worldwide market demand, intensive shrimp farming, particularly of whiteleg shrimp Litopenaeus vannamei has expanded tremendously. The present study investigated induced DNA damage in gill (GL), haemolymph (HL) and muscle (ML) cells in juveniles of L. vannamei (52.2 ± 6.4 mm; 1.78 ± 0.5 g) exposed to two sublethal concentrations, SL1 (0.36 µg l1) and SL2 (0.18 µg l1) of organophosphorus pesticide, chlorpyrifos (CPF) during 21 d of exposure (DoE). The magnitude of DNA damage (% tail DNA) as measured by comet assay at specified intervals (3, 7, 14 and 21 DoE) was found to be tissue-specific, time and dose-dependent (p < 0.05). At the end of the experiment, at 21 DoE, % tail DNA damage (mean ± SD, 53.61 ± 8.71), (49.36 ± 3.42) and (32.40 ± 4.97) was relatively higher at SL1 compared to SL2 (39.25 ± 3.90), (32.22 ± 4.21) and (22.66 ± 2.85), respectively, in GL, HL and ML cells. No significant differences in water quality parameters were found among the treated and control aquaria. The significant reduction in specific growth rates (% growth d1) observed in exposed shrimps indicated that SL concentrations of CPF negatively impacted growth in L. vannamei juveniles. A very low 96 h LC50 value (1.44 µg l1) indicated sensitivity of L. vannamei juveniles to CPF, which could be used as a bioindicator species for assessing pesticide pollution. The study results highlight the implications in extending the farming of L. vannamei to low-saline inland areas adjacent to traditional agricultural fields.