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Aquaculture Environment Interactions

    AEI prepress abstract   -  DOI:

    Effects of ocean acidification on toxicity of two trace metals on two marine molluscs in their early life stages

    Xiaoyu Guo, Miaoqin Huang, Bo Shi, Weiwei You, Caihuan Ke*

    *Corresponding author:

    ABSTRACT: Ocean acidification (OA) is usually thought to change the speciation of trace metals and to increase the concentrations of free metal ions, hence elevating the metal bioavailability. In this study, embryos of the oyster Crassostrea angulata and abalone Haliotis discus hannai were cultured under 4 pCO2 conditions (400, 800, 1500 and 2000 μatm) with Cu and Zn added at each pCO2 level. Fertilization was measured at 2 hours post-fertilization (hpf), while larval deformation and larval shell length were measured at 24 hpf. Our results show that OA can alleviate the inhibition effects of Cu and Zn on fertilization of C. angulata by 86.1 and 26.4% respectively as well as the effects of Zn on fertilization of H. discus hannai by 43.7%. However, OA enhanced the inhibitory effect of Cu on fertilization of H. discus hannai by 34.7%. OA enhanced the toxic effect of Cu on larval normality of C. angulata by 22.0%as well as for Cu and Zn on H. discus hannai by 71.4 and 37.2% respectively. OA also enhanced the inhibitory effects of Cu and Zn on larval calcification in H. discus hannai by 8.8 and 8.6% respectively. However, no interaction effect was found in the combined treatment of OA and Cu on the calcification of C. angulata larvae. Zn inhibition of oyster larval calcification was alleviated by OA from 3.1 to 1.5%. Based on our results, metal toxicity to early development of molluscs is not always increased by rising pCO2 and depends on developmental stages, egg structure and species. This complexity suggests that caution should be taken when carrying out multiple environmental stressor tests on molluscan embryos.