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Aquaculture Environment Interactions

    AEI prepress abstract   -  DOI:

    Effects of cold and starvation on the liver of yellow drum Nibea albiƱora: histological alterations and transcriptomic analysis

    Qihui Zhu**, Hongbin Song**, Yan Zhang, Ruiyi Chen, Lu Tian, Dongdong Xu*

    *Corresponding author:

    ABSTRACT: The yellow drum Nibea albiñora is a marine ðsh of great economic value in China. Despite the effort to improve yields, the aquaculture of this species is hindered by the increase in winter-related mortalities that are associated with cold and starvation. To better understand the molecular mechanisms that regulate the stress responses in yellow drum during cold and starvation, the effect of these stresses on the liver was investigated by performing comparative analyses among the fish subjected to different temperatures and feeding strategies. The experiment lasted for 22 d and 4 groups were involved, one fed group (control) and one fasted group at 16°C, as well as one fed group and one fasted group at 8°C. Our results showed that all stress-treated groups exhibited body weight loss during the experiment, demonstrating that both cold and starvation caused growth inhibition, but only the fish in the fasted group at 16°C showed a loss in liver/body ratio, suggesting that starvation could cause mass loss in the liver while cold stress caused mass loss in both liver and other tissues. Histological alterations were observed in the liver cells from stress-treated groups, also showing mass loss in the liver during cold and starvation. Transcriptomic analysis showed that genes related to metabolism such as carbohydrate, lipid and amino acid metabolism were the most enriched differentially expressed genes during cold and starvation. These findings can help reveal the molecular mechanisms regulating the stress responses of yellow drum exposed to cold and starvation.