AB 22:141-158 (2014)  -  DOI: https://doi.org/10.3354/ab00595

Synergistic effect of UV radiation and nutrient limitation on Chlorella fusca (Chlorophyta) cultures grown in outdoor cylindrical photobioreactors

I. Malpartida1, C. G. Jerez1, M. M. Morales2, P. Nascimento3, I. Freire3, J. Ezequiel4, R. M. Rico1, E. Peralta1, J. R. Malapascua1, Y. Florez1, J. Masojidek5, R. Abdala1, F. L. Figueroa1, E. Navarro6,*

1Departamento de Ecología, Facultad de Ciencias, Universidad de Málaga, 29071 Málaga, Spain
2Department of Chemical Engineering, University of Almería, Almería, Spain
3Department Microbiology and Parasitology, Faculty of Biology-CIBUS, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain
4Departamento de Biologia and CESAM - Centro de Estudos do Ambiente e do Mar, Universidade de Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal
5Academy of Sciences, Institute of Microbiology, Opatovickýmlýn, 37981 Trěboň, Czech Republic
6Pyrenean Institute of Ecology (CSIC), Av. Montañana 1005, Zaragoza 50059, Spain
*Corresponding author:

ABSTRACT: This study assessed the interactive effects of UVR and nutrient depletion on Chlorella fusca cultures on the production and accumulation of particular biomolecules. To accomplish this, algae were grown for 5 d in outdoor thin-layer cascade cultivators under 3 nutrient treatments (full nutrients, -N and -S) and then transferred to outdoor cylindrical photobioreactors for another 5 d. Cultures were then exposed to full solar radiation (PAB) and decreased UVR. During the last 5 d, bio-optical properties, photosynthetic activity, pigments, biochemical composition and oxidative stress were assessed. Initially, nutrient depletion caused changes in productivity and cell number in a manner that affected biochemical composition. After 3 d, the percentage of lipids in the cultures under N deprivation reached values appropriate for being used as feed or food additives or for energy applications (35% of lipid content), regardless of the light conditions. A longer exposure (5 d) resulted in interactive effects of light and nutrient conditions. Specifically, PAB increased lipid content in all cases (1.3- to 2.3-fold), but particularly under S deprivation. Longer exposure to PAB also increased oxidative stress in UVR and nutrient-limited treatments (-N and -S). These results showed that the benefits expected from nutrient depletion (increase in biomolecule content e.g. lipids, carbohydrates and pigments) were modulated by the negative effects of algal UVR acclimation costs.


KEY WORDS: Bio-optic · Chlorella fusca · In vivo chlorophyll fluorescence · Photosynthetic pigments · UV radiation · Lipids · Lipid peroxidation · Proteins · Biochemical composition


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Cite this article as: Malpartida I, Jerez CG, Morales MM, Nascimento P and others (2014) Synergistic effect of UV radiation and nutrient limitation on Chlorella fusca (Chlorophyta) cultures grown in outdoor cylindrical photobioreactors. Aquat Biol 22:141-158. https://doi.org/10.3354/ab00595

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