AME 54:199-210 (2009)  -  DOI: https://doi.org/10.3354/ame01264

Annual patterns of presence and activity of marine bacteria monitored by 16S rDNA–16S rRNA fingerprints in the coastal NW Mediterranean Sea

Raphaël Lami1,2,4,*, Jean-François Ghiglione1,2, Yves Desdevises1,3, Nyree J. West1,2, Philippe Lebaron1,2

1Université Pierre Marie Curie – Paris 6, Laboratoire ARAGO, Avenue du Fontaule, BP44, 66650 Banyuls-sur-Mer, France
2CNRS, UMR 7621, Laboratoire d’Océanographie Biologique de Banyuls, Avenue du Fontaule, BP44,
66650 Banyuls-sur-Mer, France
3CNRS, UMR 7628, Modèles en Biologie Cellulaire et Evolutive, Avenue du Fontaule, BP44, 66650 Banyuls-sur-Mer, France
4Present address: College of Marine and Earth Studies, University of Delaware, Lewes, Delaware 19958, USA
*Email:

ABSTRACT: The annual dynamics of the bacterial community structure and its activity remain poorly studied in marine environments. Our goal was to gain new insights into the year-long bacterial dynamics and activity in correlation with environmental changes in the NW Mediterranean Sea. To accomplish this goal, we combined 16S rDNA versus 16S rRNA fingerprints and environmental variables using multivariate analyses. A clone library was constructed to determine to which bacterial taxa the major ribotypes were related. Our results revealed similar environmental controls of bacterial community structure at both the DNA and RNA levels. The 16S rRNA signal of several ribotypes differed relative to their 16S rDNA counterpart, suggesting that the members of the community have either a higher or lower activity than their relative abundance would suggest. Such differences included the dominant ribotypes observed in the fingerprints, such as Roseobacter, Synechococcus, SAR11 and SAR116. All these results give support to the 16S rDNA and 16S rRNA fingerprinting approach to monitor bacterial community structure and activity in marine environments.


KEY WORDS: Bacteria · 16S rDNA – 16S rRNA · Bacterial activity · CE-SSCP · Canonical correspondence analysis · Mediterranean Sea · Time series


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Cite this article as: Lami R, Ghiglione JF, Desdevises Y, West NJ, Lebaron P (2009) Annual patterns of presence and activity of marine bacteria monitored by 16S rDNA–16S rRNA fingerprints in the coastal NW Mediterranean Sea. Aquat Microb Ecol 54:199-210. https://doi.org/10.3354/ame01264

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