DAO 103:121-132 (2013)  -  DOI: https://doi.org/10.3354/dao02570

Involvement of the cell-specific pigment genes pks and sult in bacterial defense response of sea urchins Strongylocentrotus intermedius

Konstantin V. Kiselev1,*, Natalya V. Ageenko2,3, Valeria V. Kurilenko4

1Laboratory of Biotechnology, Institute of Biology and Soil Science, Far Eastern Branch of Russian Academy of Sciences, Stoletija Str. 159, Vladivostok 690022, Russia
2Laboratory of Cytotechnology, A.V. Zhirmunsky Institute of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences, Palchevsky St. 17, 690059 Vladivostok, Russia
3The School of Natural Sciences, Institute of Chemistry and Applied Ecology, Far Eastern Federal University, 690090 Vladivostok, Russia
4Laboratory of Microbiology, Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, Vladivostok 690022, Russia

ABSTRACT: Bacterial infections are one of the most important problems in mass aquaculture, causing the loss of millions of juvenile organisms. We isolated 22 bacterial strains from the cavity fluid of the sea urchin Strongylocentrotus pallidus and used phylogenetic analysis based on 16S rRNA gene sequences to separate the bacterial strains into 9 genera (Aliivibrio, Bizionia, Colwellia, Olleya, Paenibacillus, Photobacterium, Pseudoalteromonas, Shewanella, and Vibrio). Incubating Strongylocentrotus intermedius larvae with a strain from each of the 9 bacterial genera, we investigated the viability of the larvae, the amount of pigment cells, and the level of polyketide synthase (pks) and sulfotransferase (sult) gene expression. Results of the assay on sea urchin development showed that all bacterial strains, except Pseudoalteromonas and Bizionia, suppressed sea urchin development (resulting in retardation of the embryos’ development with cellular disorders) and reduced cell viability. We found that pks expression in the sea urchin larvae after incubation with the bacteria of 9 tested genera was significantly increased, while the sult expression was increased only after the treatment with Pseudoalteromonas and Shewanella. Shikimic acid, which is known to activate the biosynthesis of naphthoquinone pigments, increased the tolerance of the sea urchin embryos to the bacteria. In conclusion, we show that the cell-specific pigment genes pks and sult are involved in the bacterial defense response of sea urchins.


KEY WORDS: Echinochrome · Marine bacteria · Sea urchin ·


Full text in pdf format 
Cite this article as: Kiselev KV, Ageenko NV, Kurilenko VV (2013) Involvement of the cell-specific pigment genes pks and sult in bacterial defense response of sea urchins Strongylocentrotus intermedius. Dis Aquat Org 103:121-132. https://doi.org/10.3354/dao02570

Export citation
Mail this link - Contents Mailing Lists - RSS
- -