DAO 106:273-274 (2013)  -  DOI: https://doi.org/10.3354/dao02681

COMMENT
PCR testing for diagnosis of Ichthyophonus hoferi: Comment on Hamazaki et al. (2013)

S. E. LaPatra1,*, R. M. Kocan2,*

1Research Division, Clear Springs Foods, PO Box 712, Buhl, Idaho 83316, USA
2Professor Emeritus, School of Aquatic and Fishery Science, University of Washington, Seattle, Washington 98195, USA

ABSTRACT: It is our opinion that Hamazaki et al. (2013; Dis Aquat Org 105:21–25) overstate the usefulness of PCR as a field diagnostic technique and underestimate the accuracy and utility of in vitro explant culture. In order for field diagnostic studies to be meaningful they should accurately and dependably identify the infected individuals within a population, both subclinical and clinical cases. Although explant culture, like most techniques, can miss some infected individuals, ‘false positives’ are impossible, unlike for cPCR based methodologies.


KEY WORDS: Ichthyophonus hoferi · Explant culture · Polymerase chain reaction · Diagnosis · Confirmation


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Cite this article as: LaPatra SE, Kocan RM (2013) PCR testing for diagnosis of Ichthyophonus hoferi: Comment on Hamazaki et al. (2013). Dis Aquat Org 106:273-274. https://doi.org/10.3354/dao02681

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