DAO 106:275-276 (2013)  -  DOI: https://doi.org/10.3354/dao02682

REPLY COMMENT
PCR testing for diagnosis of Ichthyophonus hoferi: Reply to LaPatra & Kocan (2013)

Toshihide Hamazaki1,*, Eryn Kahler1, Bonnie M. Borba2, Tamara Burton1

1Alaska Department of Fish and Game, Division of Commercial Fisheries, 333 Raspberry Road, Anchorage, Alaska 99518, USA
2Alaska Department of Fish and Game, Division of Commercial Fisheries, 1300 College Road, Fairbanks, Alaska 99701, USA

ABSTRACT: LaPatra & Kocan (2013) critiqued our paper Hamazaki et al. (2013; Dis Aquat Org 105:21–25) for data not supporting the conclusions of ‘PCR testing is as accurate as culture…’, but they neither pointed out what part of our data did not support our conclusion, nor did they provide any contrary scientific evidence supporting their argument that PCR testing is less accurate than culture. In the absence of any contradictory data, we stand by our data and our conclusion: PCR test is as suitable as culture as a diagnostic and field surveillance tool.


KEY WORDS: Ichthyophonus hoferi · Explant culture · Polymerase chain reaction · Diagnosis · Confirmation


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Cite this article as: Hamazaki T, Kahler E, Borba BM, Burton T (2013) PCR testing for diagnosis of Ichthyophonus hoferi: Reply to LaPatra & Kocan (2013). Dis Aquat Org 106:275-276. https://doi.org/10.3354/dao02682

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