DAO 110:201-211 (2014)  -  DOI: https://doi.org/10.3354/dao02756

Rearing practices identified as risk factors for ostreid herpesvirus 1 (OsHV-1) infection in Pacific oyster Crassostrea gigas spat

Julien Normand1,2,*, Jean-Louis Blin1,3, Aude Jouaux1,4,5 

1Centre de Référence sur l’Huître, Université de Caen Basse Normandie, Caen, France
2IFREMER LERN Port en Bessin, Station de Port-en-Bessin, Avenue Général de Gaulle, 14520 Port-en-Bessin, France
3Synergie Mer Et Littoral, Zone conchylicole, 50 560 Blainville sur mer, France
4Université de Caen Basse-Normandie, ‘Biologie des Organismes Marins et des Ecosystèmes Associés’ (BioMEA), IBFA, SFR ICORE, 14032 Caen Cedex, France
5CNRS INEE, BioMEA, Caen, France
*Corresponding author:

ABSTRACT: Early detection of Pacific oyster spat infected with ostreid herpesvirus 1 (OsHV-1) could prevent introduction of OsHV-1-infected individuals into farming areas or onshore rearing facilities, thus reducing the risk of infection of naïve oysters in such production systems. Experiments were conducted on several hundred oyster spat provided by producers in order to examine whether early rearing practices could be considered as potential risk factors for (1) OsHV-1 infection as detected by molecular methods and (2) spat mortality experimentally induced through thermal challenge. Spat groups collected on oyster beds and hatchery spat reared in growout areas during summer exhibited higher viral DNA contamination and mortalities during the trial than spat kept in onshore rearing facilities. Quantification of viral DNA before and during the trial showed that infection prevalence and intensity changed over time and revealed latent infection initially unsuspected in 3 of 10 groups. Thermal challenge induced a clear increase in the probability of detecting infected individuals, particularly for groups exhibiting significant prevalence of OsHV-1-contaminated spat prior to the challenge. The use of detection methods are discussed in relation to early rearing practices and disease control strategies.


KEY WORDS: Oyster spat · Epidemiology · OsHV-1 · qPCR · Thermal challenge


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Cite this article as: Normand J, Blin JL, Jouaux A (2014) Rearing practices identified as risk factors for ostreid herpesvirus 1 (OsHV-1) infection in Pacific oyster Crassostrea gigas spat. Dis Aquat Org 110:201-211. https://doi.org/10.3354/dao02756

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