DAO 113:215-226 (2015)  -  DOI: https://doi.org/10.3354/dao02834

Monoclonal antibody against a putative myristoylated membrane protein encoded by grouper iridovirus 59L gene

Zhi-Yu Chen1, Pinwen Peter Chiou1,2, Chian-Jiun Liou3,4, Yu-Shen Lai1,*

1Department of Biotechnology and Animal Science, National Ilan University, Yilan, Taiwan
2Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan
3Department of Nursing, Chang Gung University of Science and Technology, Taoyuan, Taiwan
4Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, Taoyuan, Taiwan
*Corresponding author:

ABSTRACT: Groupers (Epinephelus spp.) are economically important fish species worldwide, and ranaviruses are major viral pathogens causing heavy economic losses in grouper aquaculture. In this study, the 59L gene of grouper iridovirus (GIV-59L) was cloned and characterized. This gene is 1521 bp and encodes a protein of 506 amino acids with a predicted molecular mass of 53.9 kDa. Interestingly, GIV-59L and its homologs are found in all genera of the family Iridoviridae. A mouse monoclonal antibody specific for the C-terminal domain (amino acid positions 254–506) of the GIV-59L protein, GIV-59L(760-1518)-MAb-21, was produced and proved to be well suited for use in a number of GIV immunoassays. RT-PCR, Western blotting, and cycloheximide and cytosine arabinoside drug inhibition analyses indicated that GIV-59L is a viral late gene in GIV-infected grouper kidney cells. Immunofluorescence analysis revealed that GIV-59L protein mainly accumulates in the cytoplasm of infected cells and is finally packed into a whole virus particle. The GIV-59L(760-1518)-MAb-21 characterized in this study could have widespread application in GIV immunodiagnostics and other research on GIV. In addition, the results presented here offer important insights into the pathogenesis of GIV.


KEY WORDS: Grouper iridovirus 59L · Monoclonal antibody · C-terminal domain · Immunodiagnostics


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Cite this article as: Chen ZY, Chiou PP, Liou CJ, Lai YS (2015) Monoclonal antibody against a putative myristoylated membrane protein encoded by grouper iridovirus 59L gene. Dis Aquat Org 113:215-226. https://doi.org/10.3354/dao02834

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