DAO 115:87-92 (2015)  -  DOI: https://doi.org/10.3354/dao02873

NOTE
Specific PCR for Myxobolus arcticus SSU rDNA in juvenile sockeye salmon Oncorhynchus nerka from British Columbia, Canada

Amelia Mahony1, Sarah Fraser1, David B. Groman2, Simon R. M. Jones1,*

1Pacific Biological Station, Fisheries & Oceans Canada, 3190 Hammond Bay Rd., Nanaimo, British Columbia V9T 6N7, Canada
2Atlantic Veterinary College, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3, Canada
*Corresponding author:

ABSTRACT: A PCR for the specific detection of the salmon brain parasite Myxobolus arcticus (Pugachev and Khokhlov, 1979) was developed using primers designed to amplify a 1363 base pair fragment of the small subunit rDNA. The assay did not amplify DNA from 5 other Myxobolus species or from 7 other myxozoan species belonging to 5 other genera. For juvenile sockeye salmon Oncorhynchus nerka (Walbaum) collected from Chilko Lake, British Columbia (BC), Canada, in 2011, the prevalence by PCR was 96%, in contrast to 71% by histological examination of brain tissue. In 2010, the histological prevalence was 52.5%. Sequence identity between M. arcticus from Chilko Lake and other sites in BC ranged from 99.7 to 99.8% and was 99.6% for a Japanese sequence. In contrast, an M. arcticus sequence from Norway shared 95.3% identity with the Chilko Lake sequence, suggesting misidentification of the parasite. Chilko Lake sockeye salmon were previously reported free of infection with M. arcticus, and more research is required to understand the processes involved in the local and global dispersion of this parasite.


KEY WORDS: Myxozoan · Diagnostics · Salmonid · Chilko Lake · Small subunit


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Cite this article as: Mahony A, Fraser S, Groman DB, Jones SRM (2015) Specific PCR for Myxobolus arcticus SSU rDNA in juvenile sockeye salmon Oncorhynchus nerka from British Columbia, Canada. Dis Aquat Org 115:87-92. https://doi.org/10.3354/dao02873

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