DAO 115:233-244 (2015)  -  DOI: https://doi.org/10.3354/dao02892

Identification and genetic characterization of Piscirickettsia salmonis in native fish from southern Chile

Sergio Contreras-Lynch1, Paola Olmos1, Augusto Vargas2,3, Jaime Figueroa3,4, Roxana González-Stegmaier2,3, Ricardo Enríquez2, Alex Romero2,3,*

1Instituto de Fomento Pesquero, Puerto Montt 5480000, Chile
2Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia 5090000, Chile
3Facultad de Ciencias, Universidad Austral de Chile, Valdivia 5090000, Chile
4Centro FONDAP: Interdisciplinary Center for Aquaculture Research (INCAR), Universidad de Concepción, Concepción 4030000, Chile
*Corresponding author:

ABSTRACT: Piscirickettsia salmonis is the etiological agent of piscirickettsiosis, a severe disease causing high mortalities in salmonids. This bacterium has been previously identified and isolated in all cultivated salmonids in Chile and worldwide, including Salmo salar, Oncorhynchus kisutch, and O. mykiss, in addition to being found in non-salmonid species such as Dicentrarchus labrax and Atractoscion nobilis. In this study, the 16S rRNA gene and intergenic spacer ITS-1 of P. salmonis were amplified by PCR from DNA samples extracted from the native Chilean fish species Eleginops maclovinus, Odontesthes regia, Sebastes capensis, and Salilota australis. Analysis of the 16S rRNA sequences from O. regia demonstrated a close phylogenetic relationship with the 16S rRNA gene in the Chilean EM-90 strain. The 16S rRNA sequences from E. maclovinus, S. capensis, and S. australis were related to the Chilean LF-89 sequence and Scottish strains. To confirm these findings, analysis of P. salmonis ITS-1 sequences obtained from the 4 sampled native species demonstrated a high degree of identity and a close phylogenetic relationship with Chilean P. salmonis sequences, including LF-89 and EM-90. These results suggest a strong relationship between the nucleotide sequences from the 16S rRNA and ITS-1 genes amplified from native fish with those sequences described in the first P. salmonis strains to be identified and isolated in Chile.


KEY WORDS: 16S gene · ITS-1 · rDNA sequences · Piscirickettsiosis · Native fish · Salmonids


Full text in pdf format 
Cite this article as: Contreras-Lynch S, Olmos P, Vargas A, Figueroa J, González-Stegmaier R, Enríquez R, Romero A (2015) Identification and genetic characterization of Piscirickettsia salmonis in native fish from southern Chile. Dis Aquat Org 115:233-244. https://doi.org/10.3354/dao02892

Export citation
Mail this link - Contents Mailing Lists - RSS
- -