DAO 124:223-232 (2017)  -  DOI: https://doi.org/10.3354/dao03120

Isolation of Bdellovibrio and like organisms and potential to reduce acute hepatopancreatic necrosis disease caused by Vibrio parahaemolyticus 

Jetnapang Kongrueng1, Pimonsri Mitraparp-arthorn1, Khotchawan Bangpanwimon1, William Robins2, V. Vuddhakul1,*, John Mekalanos

1Food Safety and Health Research Unit, Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Thailand
2Dept. of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115, USA
*Corresponding author:

ABSTRACT: Acute hepatopancreatic necrosis disease, a severe disease of shrimp, is caused by Vibrio parahaemolyticus (AHPND Vp), a halophilic bacterium harboring a plasmid that contains toxin genes homologous to Photorhabdus insect-related toxins. We obtained 9 isolates of Bdellovibrio and like organisms (BALOs) from water and sediment samples in Thailand. Using 16S rRNA sequencing, all of the organisms were identified as Bacteriovorax spp. and were able to attack all tested AHPND Vp isolates. In addition, their various susceptible hosts, including Gram-positive and Gram-negative bacteria, were observed. The optimal ratio for interaction between the Bacteriovorax isolate BV-A and AHPND Vp was determined to be 1:10. The suitable conditions applied for co-culture between BV-A and AHPND Vp were 30°C, 2% NaCl, and pH 7.6. The capability of BV-A to reduce numbers of AHPND Vp in vitro was observed in co-culture after incubation for 2 d and continued until the end of the incubation period. In vivo, BV-A was able to reduce mortality of shrimp post-larvae infected with AHPND Vp. In addition, BV-A significantly decreased the formation of biofilm by AHPND Vp. These findings provide evidence for using Bacteriovorax as a biocontrol of AHPND Vp in shrimp aquaculture.


KEY WORDS: Bdellovibrio and like organisms · BALOs · Bacteriovorax · Vibrio parahaemolyticus · Acute hepatopancreatic necrosis disease · AHPND · Shrimp


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Cite this article as: Kongrueng J, Pimonsri Mitraparp-arthorn P, Bangpanwimon K, Robins W, Vuddhakul V, Mekalanos J (2017) Isolation of Bdellovibrio and like organisms and potential to reduce acute hepatopancreatic necrosis disease caused by Vibrio parahaemolyticus . Dis Aquat Org 124:223-232. https://doi.org/10.3354/dao03120

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