DAO 125:199-206 (2017)  -  DOI: https://doi.org/10.3354/dao03150

Prevalence, diversity and co-occurrence of the white spot syndrome virus, monodon baculovirus and Penaeus stylirostris densovirus in wild populations of Penaeus monodon in the Philippines

Fredmoore L. Orosco1,2, Arturo O. Lluisma1,*

1Marine Genomics and Molecular Genetics Laboratory, Marine Science Institute, University of the Philippines Diliman, Quezon City 1101, Phillippines
2Institute of Biology, University of the Philippines Diliman, Quezon City 1101, Phillippines
*Corresponding author:

ABSTRACT: The farming of the black tiger shrimp Penaeus monodon in the Philippines relies on wild broodstock. PCR was thus used to determine the prevalence of white spot syndrome virus (WSSV), monodon baculovirus (MBV) and Penaeus stylirostris densovirus (PstDV) in a total of 178 shrimp from 6 geographically disparate locations where broodstock are captured for use in hatcheries. PCR amplicons were also sequenced to identify phylogenetic relationships of the virus haplotypes detected. Shrimp from southeastern Luzon (Camarines Norte) had the highest prevalence of each of the 3 viruses and were frequently co-infected with 2 or more viruses. No viruses were detected in shrimp from northwestern Luzon (Pangasinan). MBV was most prevalent and PstDV strains displayed the most genetic diversity. WSSV was detected at 3 sites, and a VP28 gene sequence examined was invariant and consistent with strains found in many countries, including Thailand, China, Japan, Korea, Indonesia, Iran, Brazil and Mexico. WSSV open reading frame 94 gene sequence analysis identified location-specific repeat types. MBV sequences were dissimilar to haplotypes detected in India. PstDV sequences were diverse and included 2 lineages detected either in Australia or in the United States, Ecuador, Taiwan, China and Vietnam. The PCR data confirmed that WSSV, MBV and PstDV are endemic in P. monodon in the Philippines but that populations at some locations might remain free of infection.


KEY WORDS: White spot syndrome virus · WSSV · Penaeus stylirostris densovirus · PstDV · Monodon baculovirus · MBV · Penaeus monodon · Prevalence · Diversity · Philippines


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Cite this article as: Orosco FL, Lluisma AO (2017) Prevalence, diversity and co-occurrence of the white spot syndrome virus, monodon baculovirus and Penaeus stylirostris densovirus in wild populations of Penaeus monodon in the Philippines. Dis Aquat Org 125:199-206. https://doi.org/10.3354/dao03150

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