DAO 126:75-81 (2017)  -  DOI: https://doi.org/10.3354/dao03152

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Comparison of PCR methods for the detection of genetic variants of carp edema virus

Mikolaj Adamek1,*, Marek Matras2, Verena Jung-Schroers1, Felix Teitge1, Max Heling1, Sven M. Bergmann3, Michal Reichert2, Keith Way4, David M. Stone4, Dieter Steinhagen1

1Fish Disease Research Unit, Institute for Parasitology, University of Veterinary Medicine, Bünteweg 17, 30559 Hannover, Germany
2Laboratory of Fish Diseases, National Veterinary Research Institute, Partyzantów 57, 24-100 Puławy, Poland
3Institute of Infectology, Friedrich-Loeffler-Institut, Südufer 10, 17498 Greifswald-Insel Riems, Germany
4Centre for Environment, Fisheries and Aquaculture Science (CEFAS), Weymouth, Dorset, DT4 8UB, UK
*Corresponding author:

ABSTRACT: The infection of common carp and its ornamental variety, koi, with the carp edema virus (CEV) is often associated with the occurrence of a clinical disease called ‘koi sleepy disease’. The disease may lead to high mortality in both koi and common carp populations. To prevent further spread of the infection and the disease, a reliable detection method for this virus is required. However, the high genetic variability of the CEV p4a gene used for PCR-based diagnostics could be a serious obstacle for successful and reliable detection of virus infection in field samples. By analysing 39 field samples from different geographical origins obtained from koi and farmed carp and from all 3 genogroups of CEV, using several recently available PCR protocols, we investigated which of the protocols would allow the detection of CEV from all known genogroups present in samples from Central European carp or koi populations. The comparison of 5 different PCR protocols showed that the PCR assays (both end-point and quantitative) developed in the Centre for Environment, Fisheries and Aquaculture Science exhibited the highest analytical inclusivity and diagnostic sensitivity. Currently, this makes them the most suitable protocols for detecting viruses from all known CEV genogroups.


KEY WORDS: Carp edema virus · Koi sleepy disease · PCR · Common carp · Koi


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Cite this article as: Adamek M, Matras M, Jung-Schroers V, Teitge F and others (2017) Comparison of PCR methods for the detection of genetic variants of carp edema virus. Dis Aquat Org 126:75-81. https://doi.org/10.3354/dao03152

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