DAO 35:159-164 (1999)  -  doi:10.3354/dao035159

Preliminary characterization of a reovirus isolated from golden ide Leuciscus idus melanotus

M. Neukirch1,*, L. Haas2, H. Lehmann3, V. von Messeling2

1Fish Diseases Research Unit, School of Veterinary Medicine, Bünteweg 17, D-30559 Hannover, Germany
2Institute of Virology, School of Veterinary Medicine, Bünteweg 17, D-30559 Hannover, Germany
3Institute of Botany, Study group Cell Biology, School of Veterinary Medicine, Bünteweg 17, D-30559 Hannover, Germany

ABSTRACT: Some characteristics of a reovirus recently isolated from golden ide Leuciscus idus melanotus and tentatively designated as golden ide reovirus (GIRV) were determined. Spherical non- enveloped particles with an outer capsid of about 70 nm and an inner capsid of about 50 nm were observed by electron microscopy. The density of the virus determined in CsCl gradients was 1.36 g ml-1. The genome contained 11 segments of dsRNA. GIRV differed from other aquareoviruses by a slight reduction of infectivity after treatment with chloroform and by the absence of forming syncytia in cell monolayers.


KEY WORDS: Golden ide · Reovirus · Characterization


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