DAO 39:151-154 (2000)  -  doi:10.3354/dao039151

NOTE
Recovery of ranavirus dsDNA from formalin-fixed archival material

J. A. Kattenbelt, A. D. Hyatt*, A. R. Gould

CSIRO, Australian Animal Health Laboratory, PO Box 24, Geelong, Victoria 3200, Australia
*Corresponding author. E-mail:

ABSTRACT: The extraction and amplification of nucleic acid from formalin-fixed and paraffin-embedded tissues has become an important exercise in the collection of retrospective epidemiological data. A protocol is described that enables the extraction and amplification of dsDNA from fixed tissues within paraffin blocks and from specimens stored in 10% (aq) formalin. The procedure can be used for the examination of ranavirus DNA within archival tissues thereby providing valuable data for identifying the origin and tracing the spread of ranaviruses.


KEY WORDS: Iridoviruses · Ranaviruses · Polymerase chain reaction


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Cite this article as: Kattenbelt JA, Hyatt AD, Gould AR (2000) Recovery of ranavirus dsDNA from formalin-fixed archival material. Dis Aquat Org 39:151-154

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