DAO 53:231-240 (2003)  -  doi:10.3354/dao053231

Virulence of luminous vibrios to Artemia franciscana nauplii

S. A. Soto-Rodriguez1, A. Roque2, M. L. Lizarraga-Partida1, A. L. Guerra-Flores3, B. Gomez-Gil2,*

1Department of Marine Biotechnology, Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Ensenada 22860, Baja California, Mexico
2(CIAD), A.C. Mazatlán Unit for Aquaculture and Environmental Management, AP 711 Mazatlán, Sinaloa 82000, Mexico
3Universidad Autónoma de Sinaloa, Paseo Claussen s/n, Mazatlán, Sinaloa 82000, Mexico
*Corresponding author. Email:

ABSTRACT: From healthy and diseased penaeid shrimp from Asia and the Americas, 25 luminous and 2 non-luminous bacterial strains were isolated, and 14 were phenotypically identified as Vibrio harveyi; 9 isolates produced significant mortalities (45 to 80%) in Artemia franciscana nauplii at inoculation densities of 105 to 106 CFU ml-1 compared to the controls (unchallenged nauplii). The maximum number of bacteria ingested (bioencapsulated) by the Artemia nauplii varied from less than 10 to 103 CFU nauplius-1 and no significant relationship was observed between the density of bacteria inoculated, the amount of bacteria ingested, and naupliar mortality. Significant correlations were obtained between naupliar mortality and production of proteases, phospholipases or siderophores, but not between mortality and lipase production, gelatinase production, hydrophobicity or hemolytic activity. The results suggest that virulence of the strains tested was more related to the production of particular exoenzymes than to the measured colonization factors.


KEY WORDS: Pathogenicity · Luminous Vibrio · Hydrophobicity · Exoenzymes · Artemia


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