DAO 57:147-150 (2003)  -  doi:10.3354/dao057147

Vibrio alginolyticus associated with white spot disease of Penaeus monodon

Joseph Selvin1,*, A. P. Lipton2

1Department of Biotechnology, Malankara Catholic College, Mariagiri, Kaliakavilai 629 153, Kanyakumari District, India
2Marine Biotechnology Laboratory, Central Marine Fisheries Research Institute, Vizhinjam 695 521, Thiruvananthapuram, India

ABSTRACT: In February 2000, white spot disease outbreaks occurred among cultured Penaeus monodon in extensive shrimp farms on the southwest coast of India. Bacteria were isolated from infected shrimp that showed reddish body coloration and white spots in the cuticle. The isolates were screened on thiosulfate citrate bile salt sucrose (TCBS) agar plates for the selection of Vibrio species. The primary isolate (QS7) was characterized as V. alginolyticus based on morphological, biochemical and physiological characteristics. Antibiotic sensitivity tests of QS7 indicated that the isolate was highly sensitive to chloramphenicol, ciprofloxacin, nalidixic acid and streptomycin. Pathogenicity tests confirmed that the isolate was virulent for P. monodon. Based on the lethal dose (LD50) value (5 ×106 cfu per shrimp), it was inferred that shrimp weakened by white spot syndrome virus would succumb to secondary infection by QS7.


KEY WORDS: Bacterial disease · Penaeus monodon · Secondary pathogen · Vibrio · Antibiogram · Pathogenicity


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