DAO 72:179-184 (2006)  -  doi:10.3354/dao072179

Viral interference between infectious hypodermal and hematopoietic necrosis virus and white spot syndrome virus in Litopenaeus vannamei

Valérie Bonnichon1, Donald V. Lightner2, Jean-Robert Bonami 1,*

1Pathogens and Immunity, UMR 5119, CNRS-UM2, cc093, Université Montpellier 2, Place E. Bataillon, 34095 Montpellier Cedex 05, France
2Department of Veterinary Science and Microbiology, University of Arizona, 1117 E. Lowell, Tucson, Arizona 85721, USA
*Corresponding author. Email:

ABSTRACT: White spot syndrome virus (WSSV) is highly virulent and has caused significant production losses to the shrimp culture industry over the last decade. Infectious hypodermal and hematopoietic necrosis virus (IHHNV) also infects penaeid shrimp and, while being less important than WSSV, remains a major cause of significant production losses in Litopenaeus vannamei (also called Penaeus vannamei) and L. stylirostris (also called Penaeus stylirostris). These 2 viruses and their interactions were previously investigated in L. stylirostris. We report here laboratory challenge studies carried out to determine if viral interference between IHHNV and WSSV also occurs in L. vannamei, and it was found that experimental infection with IHHNV induced a significant delay in mortality following WSSV challenge. L. vannamei infected per os with IHHNV were challenged with WSSV at 0, 10, 20, 30, 40 and 50 d post-infection. Groups of naïve shrimp infected with WSSV alone died in 3 d whereas shrimp pre-infected with IHHNV for 30, 40 or 50 d died in 5 d. Real-time PCR analysis showed that the delay correlated to the IHHNV load and that WSSV challenge induced a decrease in IHHNV load, indicating some form of competition between the 2 viruses.

KEY WORDS: White spot syndrome virus · WSSV · Infectious hypodermal and hematopoietic necrosis virus · IHHNV · Litopenaeus vannamei · Viral interference

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