DAO 80:27-35 (2008)  -  DOI: https://doi.org/10.3354/dao01922

First report of a Mikrocytos-like parasite in European oysters Ostrea edulis from Canada after transport and quarantine in France

N. Gagné1,*, N. Cochennec2, M. Stephenson1, S. McGladdery3, G. R. Meyer4, S. M. Bower4

1Fisheries and Oceans Canada, Gulf Fisheries Center, PO Box 5030, Moncton, New Brunswick E1C 9B6, Canada
2IFREMER/COP, BP 7004, 98719 Taravao, Tahiti, French Polynesia
3Fisheries and Oceans Canada, National Capital Region, Ottawa, Ontario K1A 0E6, Canada
4Fisheries and Oceans Canada, Pacific Biological Station, Nanaimo, British Columbia V9R 5K6, Canada

ABSTRACT: As part of a disease resistance experiment, 112 apparently healthy European flat oysters Ostrea edulis L. were exported from Canada (Nova Scotia) into France to test their susceptibility to Bonamia ostreae infection. Twelve oysters died in transit and 17 others died within 2 wk of laboratory quarantine acclimation. All oysters were examined histologically, and the 17 that died during quarantine were assayed for microcells (Bonamia sp. and Mikrocytos mackini) using molecular techniques. A microcell parasite was detected in the connective tissue of 5 of the 112 oysters. Morphological appearance, tissue affinity and molecular characterization through PCR, in situ hybridization (ISH), fluorescence in situ hybridization (FISH) and sequencing revealed a protist related to M. mackini. This is the first report of a parasite of the genus Mikrocytos in a species belonging to the genus Ostrea from the Atlantic Ocean.


KEY WORDS: Mikrocytos · Ostrea edulis · In situ hybridization · PCR · Histology


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Cite this article as: Gagné N, Cochennec N, Stephenson M, McGladdery S, Meyer GR, Bower SM (2008) First report of a Mikrocytos-like parasite in European oysters Ostrea edulis from Canada after transport and quarantine in France. Dis Aquat Org 80:27-35. https://doi.org/10.3354/dao01922

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